LOCUS       FLAG-EAF pBacPAK8                6252 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-EAF pBacPAK8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Kong SE, Banks CA, Shilatifard A, Conaway JW, Conaway RC
  TITLE     ELL-associated factors 1 and 2 are positive regulators of RNA 
            polymerase II elongation factor ELL.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jul 19. 102(29):10094-8.
  PUBMED    16006523
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|gt12365.cn|
COMMENT     VNTNAME|FLAG-EAF pBacPAK8|
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     2..829
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             2..126
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(164..769)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        833..924
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     849..866
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             934..951
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1006..1029
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_recomb     1890..3247
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1906..3247)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      3394..3849
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3481..3500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3691..3712
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3866..3885)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3985..4007
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4045..4063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4131..4235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4236..5096
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4454..4473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5267..5855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5756..5775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6009..6026
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgcaccttt taatcaaatc ccaagatgtg tataaaccac caaactgcca aaaaatgaaa
       61 actgtcgata agctctgtcc gtttgctggc aactgcaagg gtctcaatcc tatttgtaat
      121 tattgaataa taaaacaatt ataaatgtca aatttgtttt ttattaacga tacaaaccaa
      181 acgcaacaag aacatttgta gtattatcta taattgaaaa agcgtagtta taatcgctga
      241 ggtaatattt aaaatcattt tcaaatgatt cacagttaat ttgcgacaat ataattttat
      301 tttcacataa actagacgcc ttgtcgtctt cttcttcgta ttccttctct ttttcatttt
      361 tctcctcata aaaattaaca tagttattat cgtatccata tatgtatcta tcgtatagag
      421 taaatttttt gttgtcataa atatatatgt cttttttaat ggggtgtata gtaccgctgc
      481 gcatagtttt tctgtaattt acaacagtgc tattttctgg tagttcttcg gagtgtgttg
      541 ctttaattat taaatttata taatcaatga atttgggatc gtcggttttg tacaatatgt
      601 tgccggcata gtacgcagct tcttctagtt caattacacc attttttagc agcaccggat
      661 taacataact ttccaaaatg ttgtacgaac cgttaaacaa aaacagttca cctccctttt
      721 ctatactatt gtctgcgagc agttgtttgt tgttaaaaat aacagccatt gtaatgagac
      781 gcacaaacta atatcacaaa ctggaaatgt ctatcaatat atagttgctg atatcatgga
      841 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
      901 tttgtaataa aaaaacctat aaataccatg ggtcatcatc atcaccatca cggtggtcga
      961 caagcttgga tccctgcagg cctcgagttc gaatctagaa tgccagacta caaggacgat
     1021 gatgacaagc tcgagatgaa tgggaccgca aacccgctgc tggaccgcga ggaacattgc
     1081 ctgaggctcg gggagagctt cgagaagcgg ccgcgggcct ccttccacac tattcgttat
     1141 gattttaaac cagcatctat agacacttcc tgtgaaggag agcttcaagt tggcaaagga
     1201 gatgaagtca caattacact gccacatatc cctggatcca caccacccat gactgtgttc
     1261 aaggggaaca aacggcctta ccagaaagac tgtgtgctta ttattaatca tgacactggt
     1321 gaatatgtgc tggaaaaact cagtagcagc attcaggtga agaaaacaag agctgagggc
     1381 agcagtaaaa tccaggcccg aatggaacag cagcccactc gtcctccaca gacgtcacag
     1441 ccaccaccac ctccaccacc tatgccattc agagctccaa cgaagcctcc agttggaccc
     1501 aaaacttctc ccttgaaaga taacccctca cctgaacctc agttggatga catcaaaaga
     1561 gagctgaggg ctgaagttga cattattgaa caaatgagca gcagcagtgg gagcagctct
     1621 tcagactctg agagctcttc gggaagtgat gacgatagct ccagcagtgg aggcgaggac
     1681 aatggcccag cctctcctcc gcagccttca caccagcagc cctacaacag taggcctgcc
     1741 gttgccaatg gaaccagccg gccacaagga agcaaccagc tcatgaacac cctcagaaat
     1801 gacttgcagt tgagtgagtc tggcagtgac agtgatgact agggtaccga gctcgaattc
     1861 ccgggcggcc gcttaattaa ttgatccggg ttattagtac atttattaag cgctagattc
     1921 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
     1981 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
     2041 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
     2101 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
     2161 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
     2221 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
     2281 agtgtacaat tgactcgacg taaacacgtt aaataacgct tggacatatt taacatcggg
     2341 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
     2401 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
     2461 cgcgatcaaa tttgtagttg agctttttgg aattatttct gattgcgggc gtttttgggc
     2521 gggtttcaat ctaactgtgc ccgattttaa ttcagacaac acgttagaaa gcgatggtgc
     2581 aggcggtggt aacatttcag acggcaaatc tactaatggc ggcggtggtg gagctgatga
     2641 taaatctacc atcggtggag gcgcaggcgg ggctggcggc ggaggcggag gcggaggtgg
     2701 tggcggtgat gcagacggcg gtttaggctc aaatgtctct ttaggcaaca cagtcggcac
     2761 ctcaactatt gtactggttt cgggcgccgt ttttggtttg accggtctga gacgagtgcg
     2821 atttttttcg tttctaatag cttccaacaa ttgttgtctg tcgtctaaag gtgcagcggg
     2881 ttgaggttcc gtcggcattg gtggagcggg cggcaattca gacatcgatg gtggtggtgg
     2941 tggtggaggc gctggaatgt taggcacggg agaaggtggt ggcggcggtg ccgccggtat
     3001 aatttgttca ggtttagttt gttcgcgcac gattgtgggc accggcgcag gcgccgctgg
     3061 ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg ggtggtggca attcaatatt
     3121 ataattggaa tacaaatcgt aaaaatctgc tataagcatt gtaatttcgc tatcgtttac
     3181 cgtgccgata tttaacaacc gctcaatgta agcaattgta ttgtaaagag attgtctcaa
     3241 gctcggatcg atcccgcacg ccgataacaa gccttttcat ttttactaca gcattgtagt
     3301 ggcgagacac ttcgctgtcg tcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3361 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     3421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3601 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3721 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     3841 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3901 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3961 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4021 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4501 tcggtcgccg catacactat tctcagaatg acttggttga atattcacca gtcacagaaa
     4561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6001 ccgagcgcag cgagtcagtg agcgaggaag ctcgacatgc tgaacaacaa gattaatatg
     6061 cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc gcgcggcggt
     6121 atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc gtgtgccaag
     6181 tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt
     6241 gtcggtgaag tc
//